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PAFDA Junior Scientist Microbiology | Complete Solved Paper | 23-03-2025 | TAZ Vlogs

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[0:11]The Torch Bearers by A&Z Junior Scientist Microbiology PAFDA solved Paper 23.03.2025.

[0:26]Our team, Ahmad Shah Zaman, founder and CEO, Zeshan Akram, founder and CEO.

[0:36]Screening Written Paper Questions. Polysaccharides are polymers. Which enzyme uses hydrogen as a substrate for oxygen receptor? Catalase. Screening Written Paper Questions. Which bond is present in glucose molecule? Glycosidic bond. Toxins produced by nuts and peanuts? Amylase. Screening Written Paper Questions. Mass spectrometry separate on the basis of mass to charge ratio. Enzyme helps in lowering activation energy. Virion capsid is made up of protein. Screening Written Paper Questions. Which technique help separation of oil from food products? Centrifugation. Bacterial cell can be counted by plate count method. Colony isolation from one type of culture pure colony. Screening Written Paper Questions. Which enzyme used in DNA unwinding in replication? Helicase. Which of the following is not nutrients? Water. Which technique can be used for sterilisation of sugar solution? Autoclave. Screening Written Paper Questions. If pH is 3 then what would be pOH? 11. Which enzyme uses hydrogen as a substrate? Hydrogenase. Fungi that absorb nutrients from dead organic matter are called as saprotrophs. Screening Written Paper Questions. Instrument for radiation measurement is called as Geiger counter. Which instrument separate substance based on mass to charge ratio? Mass spectrometer. Fungal association with the roots of higher plants is called Mycorrhiza. Screening Written Paper Questions. Enzyme for conversion of glucose-6-phosphate to fructose-6-phosphate phosphoglucose isomerase. Pathogen having only primary inoculum is called as Monocyclic. Enzyme that cuts DNA? Restriction Endonuclease. Screening Written Paper Questions. The term that best describes the indigenous microorganisms is commensals. Compound microscope has magnification 1500x with oil immersion lens. What would be power of ocular lens? 15x. Screening Written Paper Questions. Method used for preservation of food to reduce osmotic pressure Salting. Which carbon atom is involved in ring formation in glucose? Carbon atom C1 (the first carbon) and carbon atom C5 (the fifth carbon). Screening Written Paper Questions. In PCR, temperature during extension is 72°C. The conditions for Autoclave are 121°C and 15 PSI. Lyophilization is freeze-drying method. Screening Written Paper Questions. Organelles that contain digestive enzymes lysosomes. Which of the following is Not involved in PCR? Transformation. Purines contains Adenine & Guanine. Screening Written Paper Questions. Which of the following is type of pyrimidine? Uracil. Penicillin was discovered by Alexander Fleming. Central Dogma refers to DNA is transcribed to RNA, which is then translated into proteins. Screening Written Paper Questions. Water activity is defined as The ratio of the vapor pressure of water in a substance to the vapor pressure of pure water at the same temperature. Transgenic Organisms are organisms whose genomes have been altered by introducing foreign DNA, called a transgene, from another species. Screening Written Paper Questions. Which of the following is called as Powerhouse of Cell? Mitochondria. The cleaning of drinking water is done by Chlorination.

[5:47]Screening Written Paper Questions. Function of stomata? Helps in gaseous exchange. Different layers of liquid can be separated through Decantation. In prokaryotes proteins synthesis occur in which organelle? Ribosomes.

[6:10]Screening Written Paper Questions. Pili helps in Motility & movement. The focus of biotechnology among following is Vaccine Production. ISO 9001 also deals with Customer satisfaction. Screening Written Paper Questions. In the context of nucleic acids (DNA and RNA), an "A to U sequence" refers to A specific type of sequence where adenine (A) is replaced with uracil (U). How many ATP molecules are produced during glycolysis? 4 ATP molecules.

[6:55]Screening Written Paper Questions. For DNA hybridisation, which of the following method can be used? Southern Blotting. Which component of the compound microscope gathers and focuses light on the specimen? Condenser. Screening Written Paper Questions. In bacteria, which of the following helps in mobility? Pertritrichous flagella. External quality assessment helps to reduce errors. Which of the following can be used as Biofuel? Ethanol. Screening Written Paper Questions. Biosafety and biosecurity guidelines are given by FDA. Which enzyme is used to convert pyruvate into lactate? Lactate dehydrogenase. Which one is viral infection? Influenza. Screening Written Paper Questions. Which one is not a restriction site? All of these. The document containing the do's and don'ts related to laboratory only Good laboratory practices. Chemoautotrophs need to survive? Carbon. Screening Written Paper Questions. Hemoglobin molecules requires which metal? Iron. How many ATP molecules are generated by the breakdown of one molecule of glucose? 38 ATP molecules. What is not a PCR reagent? Monosodium Sulphate. Screening Written Paper Questions. What is the example of plant host resistance? Pathogen induced immunity. The legal restrictions that prevents the transportation of plants to stop the spread of pathogen? Quarantine. Which organization governs good laboratory practices? FDA. Screening Written Paper Questions. The ability of a pathogen to cause disease is called pathogenicity. Which of the following is not a part of virus? Cell wall. Rhizodia is linked with legumes. Screening Written Paper Questions. In host pathogen, resistance is shown by producing secondary metabolites. Bacterial adhesins are used for attachment to cell. Motility is checked by flagella staining. Screening Written Paper Questions. Which one is a purine? Adenine. What is Functional fermentation? Functional fermentation refers to the use of fermentation processes to create foods that not only provide basic nutrition but also offer specific health benefits beyond those found in the original food. Screening Written Paper Questions. Which standards governs the biosafety and biosecurity of microbiology lab? WHO Laboratory Biosafety Manual. The term biotechnology refers to Any technological application that utilizes biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific uses. Screening Written Paper Questions. Gene therapy definition? Medical approach that aims to treat or prevent diseases by correcting underlying genetic problems, often by replacing a faulty gene with a healthy one or by introducing a new gene to help fight a disease. Thank you.

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